| Primer | Species | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 31 | Methanosphaera stadtmanae | AGACTGGGATGCAAAGGACA | TGCTGTGTTGTAGTCGGCT | 58.93 | 59.25 | 292 | 128 |
100.00%
|
100.00%
|
| Primer 32 | Methanosphaera stadtmanae | TGGGATGCAAAGGACAGTGAA | TGCTGTGTTGTAGTCGGCT | 59.86 | 59.25 | 288 | 128 |
100.00%
|
100.00%
|
| Primer 33 | Methanosphaera stadtmanae | CTGGGATGCAAAGGACAGTG | TGCTGTGTTGTAGTCGGCT | 58.83 | 59.25 | 289 | 128 |
100.00%
|
100.00%
|
| Primer 34 | Methanosphaera stadtmanae | AGCCAATAGACTGGGATGCA | TGCTGTGTTGTAGTCGGCT | 58.78 | 59.25 | 299 | 128 |
100.00%
|
100.00%
|
| Primer 35 | Methanosphaera stadtmanae | CTGGGATGCAAAGGACAGTGA | TGCTGTGTTGTAGTCGGCT | 60.27 | 59.25 | 289 | 128 |
100.00%
|
100.00%
|
| Primer 36 | Methanosphaera stadtmanae | GACTGGGATGCAAAGGACAGT | TGCTGTGTTGTAGTCGGCT | 60.27 | 59.25 | 291 | 128 |
100.00%
|
100.00%
|
| Primer 37 | Methanosphaera stadtmanae | CCGCATCTGTAGGTGCAGT | TGCTGCTCCTGCACCAAT | 59.78 | 59.56 | 191 | 128 |
100.00%
|
100.00%
|
| Primer 38 | Methanosphaera stadtmanae | GGCATGTGAAGGGGTAGGA | AGTTGAGCTGTTTGTGCATCT | 58.69 | 58.42 | 156 | 127 |
100.00%
|
100.00%
|
| Primer 39 | Methanosphaera stadtmanae | GGCATGTGAAGGGGTAGGAA | AGTTGAGCTGTTTGTGCATCT | 59.38 | 58.42 | 156 | 127 |
100.00%
|
100.00%
|
| Primer 40 | Methanosphaera stadtmanae | ACAGAACCTGCTGGATGATCT | ACCCCATGTTACAACTGCCA | 58.81 | 59.52 | 229 | 127 |
100.00%
|
100.00%
|